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Structural biology is the study of the architecture and shape of biological macromolecules--proteins and nucleic acids in particular--and what causes them to have they structures they have. This subject is of
great interest to biologists, because macromolecules carry out most of the functions of a cell, and because typically it only is by coiling into a specific three-dimensional shape that they
are able to perform their functions. This shape, which is called the "tertiary structure" of a molecule, depends in a complicated way on the molecule's basic composition, or
"primary structure."
Biomolecules are too small to see in detail even with the most advanced
microscopes. The methods that structural biologists use to determine their
structures generally involving measurements on vast numbers of identical molecules at the same time. These methods include
crystallography, NMR and circular dichroism. Most often researchers use them to study the static
"native states" of macromolecules. But variations on these methods are also
used to watch nascent or denatured molecules assume or reassume their native states
(see e.g. protein folding).
A third approach that structural biologists take to understanding structure is bioinformatics to look for patterns among the diverse sequences that give rise to particular shapes. Researchers often can deduce aspects of the structure of
integral membrane proteins based on the membrane topology predicted by hydrophobicity
analysis. See: protein structure
prediction.
See also: primary structure, secondary structure, tertiary structure, quaternary
structure, structural domain, structural motif, protein subunit, cooperativity, chaperonin, structural genomics
External links
- Nature: Structural &
Molecular Biology magazine website
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