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Molecular biology is the study of biology at a molecular level. The field overlaps with other areas of biology, particularly genetics and biochemistry. Molecular biology chiefly concerns itself with understanding the interactions between the
various systems of a cell, including the interrelationship of DNA, RNA and protein synthesis and learning how these interactions
are regulated.
Writing in Nature, W.T. Astbury described molecular
biology as:
"... not so much a technique as an approach, an approach from the viewpoint of the so-called basic sciences with the leading
idea of searching below the large-scale manifestations of classical biology for the corresponding molecular plan. It is concerned
particularly with the forms of biological molecules and ..... is predominantly three-dimensional and structural - which does not
mean, however, that it is merely a refinement of morphology - it must at the same time inquire into genesis and function"
[Nature 190, 1124 (1961)]
Relationship to other "molecular-scale" biological sciences
Researchers in molecular biology use specific techniques native to molecular biology (see Techniques section later in
article), but increasingly combine these with techniques and ideas from genetics,
biochemistry and biophysics. There is not a hard-line between these disciplines as there once was. The following figure is a
schematic that depicts one possible view of the relationship between the fields:
Schematic relationship between biochemistry, genetics and molecular biology
- Biochemistry is the study of molecules (e.g. proteins) in the absence of
the rest of the organism. Biochemists take an organism or cell and dissect it into its molecular components, such as enzymes, lipids and DNA, and reconstitute them in test
tubes (in vitro).
- Genetics is the study of the effect of genetic differences on organisms. Often this can be inferred by the absence
of a normal component (e.g. one gene). The study of "mutants" – organisms which lack one or more functional components with respect to the so-called "wild type" or normal phenotype. Genetic interactions such as epistasis can often confound simple interpretations of such "knock-out" studies.
- Molecular biology is the study of molecular underpinnings of the process of replication, transcription and
translation of the genetic material. The central dogma of molecular biology
where genetic material is transcribed into RNA and then translated into protein, despite being an oversimplified picture of
molecular biology, still provides a good starting point for understanding the field. This picture, however, is undergoing
revision in light of emerging novel roles for RNA.
Much of the work in molecular biology is quantitative, and recently much work has been done at the interface of molecular
biology and computer science in bioinformatics and computational biology. As of the early 2000s, the study of gene structure and function, molecular genetics, has been amongst the most prominent sub-field of molecular biology.
Increasingly many other fields of biology focus on molecules, either directly studying their interactions in their own right
such as in cell biology and developmental biology, or indirectly, where the techniques of molecular biology are used to infer
historical attributes of populations or species, as in fields in evolutionary biology such as population genetics and phylogenetics. There is also a long tradition of studying biomolecules "from the ground up" in biophysics.
Techniques of molecular biology
Since the late 1950s and early 1960s,
molecular biologists have learned to characterise, isolate, and manipulate the molecular components of cells and organisms. These
components include DNA, the repository of genetic information; RNA, a close relative of DNA whose functions range from serving as a temporary working copy of DNA to actual structural
and enzymatic functions as well as a functional and structural part of the translational apparatus; and proteins, the major structural and enzymatic type of molecule
in cells.
Expression cloning
One of the most basic techniques of molecular biology to study protein function is expression cloning. In this technique, DNA
coding for a protein of interest is cloned (using PCR
and/or restriction enzymes) into a plasmid (known as an expression vector). This plasmid may have special promoter elements to drive production
of the protein of interest, and may also have antibiotic resistance markers to help follow the plasmid.
This plasmid can be inserted into either bacterial or animal cells. Introducing DNA into bacterial cells is called transformation, and can be effected by several methods, including electroporation, microinjection and chemically. Introducing DNA into eukaryotic cells, such as animal cells, is called transfection. Several different transfection technqiues are
available, including calcium phosphate transfection, liposome
transfection, and proprietary transfection reagents such as Fugene. DNA can also be introduced into cells using viruses as a
carrier. In such cases, the technique is called viral transduction, and the cells are said to be transduced.
In either case, DNA coding for a protein of interest is now inside a cell, and the protein can now be expressed.. A variety of
systems, such as inducible promoters and specific cell-signaling factors, are available to help express the protein of interest
at high levels. Large quantities of a protein can then be extracted from the bacterial or eukaryotic cell. The protein can be
tested for enzymatic activity under a variety of situations, the protein may be crystallized so its tertiary structure can be
studied, or, in the pharmaceutical industry, the activity of new drugs against the protein can be studied.
Polymerase chain reaction (PCR)
Main article: Polymerase chain
reaction
The polymerase chain reaction is an extremely
versatile technique for copying DNA. In brief, PCR allows a single DNA sequence to be copied (millions of times), or altered in
predetermined ways. For example, PCR can be used to introduce restriction enzyme sites, or to mutate (change) particular bases of
DNA. PCR can also be used to determine whether a particular DNA fragment is found in a cDNA
library.
Gel electrophoresis
Main article: Gel electrophoresis
Gel electrophoresis is one of the principal tools of molecular biology. The basic principle is that DNA, RNA, and proteins can
all be separated using an electric field. In agarose gel electrophoresis, DNA and RNA can be separated based on size by running the DNA
through an agarose gel. Proteins can be separated based on size using an SDS-PAGE gel. Proteins can also be separated based on
their electric charge, using what is known as an isoelectric
gel...
Western blotting and immunochemistry
Main article: Western blot
Antibodies to most proteins can be created by injecting small amounts of the protein into an animal such as a mouse, rabbit,
sheep, or donkey. These antibodies can be used for a variety of analytical and preprative techniques.
In Western blotting, proteins are first separated by size, in a thin gel
sandwiched between two glass plates. This technique is called SDS-PAGE (for Sodium Dodecyl Sulfate Poly-Acrylamide Gel
Electrophoresis). The proteins in the gel are then transferred to a PVDF, nitrocellulose, nylon or other support membrane. This
membrane can then be probed with solutions of antibodies. Antibodies that specifically bind to the protein of interest can then
be visualized by a variety of techniques, including chemoluminescence or radioactivity.
Antibodies can also be used to purify proteins. Antibodies to a protein are generated and are often then coupled to "beads".
After the antibody has bound to the protein of interest, this antibody-protein complex can be separated from all other proteins
by centrifugation. During centrifugation, the beads, to which the antibody is coupled, will pellet (bringing the protein of
interest down with it) whereas all other proteins will remain in the solution. Alternatively, antibodies coupled to a solid
support matrix like Sephadex or Sepharose beads, for example, can be used to remove a protein of interest from a complex
solution. After washing unbound and non-specifically bound materials away from the "beads", the protein of interest is then
eluted from the matrix, usually by adding a solution with a high salt concentration, or by varying the pH of the solution in
which the matrix is contained. The beads can either be suspended in solution (batch processing) or packed into a tube (column
processing).
History
Molecular biology was established in the 1930s, the term was first coined by Warren Weaver in 1938 however. Warren was
director of Natural Sciences for the Rockefeller
Foundation at the time and believed that biology was about to undergo a period of significant change given recent advances in
fields such as X-ray crystallography. He therefore
channeled significant amounts of (Rockefellor Institute) money into biological fields.
Further reading
- Keith Roberts, Martin Raff, Bruce Alberts, Peter Walter, Julian Lewis and Alexander Johnson, Molecular Biology of the
Cell, 4th Edition, Routledge, March, 2002, hardcover, 1616 pages, 7.6 pounds, ISBN 0815332181
Related topics
Notable molecular biologists
See also
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